The ability of cells to sense and respond to their microenvironment is essential for all aspects of an organism (development, life, regeneration). Many signaling pathways are regulated by the joint action of soluble factors (such as growth factors) and extracellular matrix-bound ligands. In this context, our project aims at developing a new biomimetic platform combining microfluidics systems to control gradients of soluble molecules, with an innovative substrate allowing to reversibly present or hide a ligand upon light illumination. Our strategy is to combine the use of a PLL-g-PNIPAM copolymer approach that allows subcellular modulation of ligand accessibility upon temperature switch, with the generation of photothermal gradients in the vicinity of gold nanoparticles by thermoplasmonic excitation.

Publié le 13/04/2021